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人羊膜细胞.

简要描述:CCL-25 WISH 人羊膜细胞.,原代细胞|细胞系|细胞株|菌种;细胞库管理规范,
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  • 产品型号:WISH
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  • 更新时间:2024-03-11
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CCL-25 WISH 人羊膜细胞.

ATCC® Number: CCL-25™ Price: $323.00

Additional information about this cell line

Designations: WISH

Depositors: L Hayflick

Biosafety Level: 2

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial

CCL-25 WISH 人羊膜细胞.

Source: Organ: HeLa contaminant

Cellular Products: keratin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Virus Susceptibility: poliovirus 1, 2; adenovirus 3; vesicular stomatitis (Indiana)

Reverse Transcript: negative

DNA Profile (STR): Amelogenin: X

CSF1PO: 9,10

D13S317: 13.3

D16S539: 9,10

D5S818: 11,12

D7S820: 8,12

THO1: 7

TPOX: 8,12

vWA: 16,18

Isoenzymes: G6PD, A

HeLa Markers: Y

Comments: This line was originally thought to be derived from normal amnion, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination.

The cells are positive for keratin by immunoperoxidase staining.

NOTE: Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination

Propagation: ATCC complete growth medium: Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%

Temperature: 37.0°C

Subculturing: Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:10 is recommended

Medium Renewal: Twice per week

Remove medium, add fresh 0.25% trypsin solution for 3 to 5 minutes, remove trypsin and let culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 22153: Hayflick L. The establishment of a line (WISH) of human amnion cells in continuous c*tion. Exp. Cell Res. 23: 14-20, 1961. PubMed: 13712490

26034: . . Am. J. Dis. Child. 103: 460, 1962.




CCL-25 WISH人羊膜细胞。

ATCC®编号:CCL-25™价格:323.00美元

有关此细胞系的其他信息

名称:WISH

存款人:L Hayflick

生物安全级别:2

运送:冷冻

中等和血清:见传播

生长特性:贴壁

生物体:智人(人)

形态:上皮

CCL-25 WISH

CCL-25 WISH人羊膜细胞。

来源:器官:HeLa污染物

蜂窝产品:角蛋白

许可证/表格:除上述MTA之外,转移此ATCC材料可能需要其他ATCC和/或管理许可证。购买ATCC材料的任何人终都需要负责获得许可。请点击此处了解有关装运到您所在地的具体要求的信息。

病毒易感性:脊髓灰质炎病毒1,2;腺病毒3;水泡性口炎(印第安纳州)

反转录:否定

DNA图谱(STR):釉原蛋白:X.

CSF1PO:9,10

D13S317:13.3

D16S539:9,10

D5S818:11,12

D7S820:8,12

THO1:7

TPOX:8,12

vWA:16,18

同工酶:G6PD,A

HeLa标记:Y

评论:这条线

zi初被认为是源自正常的羊膜,但后来发现,基于同工酶分析,HeLa标记染色体和DNA指纹分析已经通过HeLa细胞污染建立。

通过免疫过氧化物酶染色,细胞对角蛋白呈阳性。

注意:此行的细胞含有HeLa标记染色体,并且是通过HeLa污染衍生的

繁殖:ATCC*生长培养基:Earle's BSS中的低必需培养基(Eagle),含有非必需氨基酸和1mM丙酮酸钠,90%;胎牛血清,10%

温度:37.0℃

传代培养:传代培养比例:建议传代比例为1:2至1:10

中等更新:每周两次

取出培养基,加入新鲜的0.25%胰蛋溶液3到5分钟,去除,让培养物在室温下静置5-10分钟。加入新鲜培养基,吸入并分装到新的培养瓶中。

保存:培养基95%; DMSO,5%

相关产品:推荐培养基(不含ATCC培养基描述的额外补充剂或血清):ATCC 30-2003

推荐血清:ATCC 30-2020

参考文献:22153:Hayflick L.在连续培养中建立人羊膜细胞系(WISH)。进出口。细胞研究。 23:14-20,1961。PubMed:13712490

26034:。 。上午。 J. Dis。儿童。 103:460,1962。


















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